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View this table:
Table 1.

Rhythm Categories

Shockable rhythms: lethal rhythms that terminate in the patient’s death unless defibrillation is delivered very quickly. These rhythms include coarse ventricular fibrillation (VF) and rapid ventricular tachycardia (VT) and are always (VF) or almost always (rapid VT) associated with a pulseless, unresponsive patient.

Nonshockable rhythms: benign (or even normal) rhythms that must not be shocked, especially in patients with a pulse, because no benefit will follow and deterioration in rhythm may result. Nonshockable rhythms include normal sinus rhythm, supraventricular tachycardias, sinus bradycardia, atrial fibrillation and flutter, heart block, idioventricular rhythms, premature ventricular contractions, and other rhythms accompanied by a palpable pulse and/or occurring in a conscious patient. To maximize safety in the event of misapplication of the device/electrodes, asystole is included in this group. The AHA Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiac Care discourage shocks for asystole. 1

Intermediate rhythms: Rhythms for which the benefits of defibrillation are limited or uncertain. These include fine VF (associated with pulselessness and low survival rates) and VT that does not meet all criteria for inclusion in the shockable VT rhythm category.

Various cardiac rhythms are categorized in Table 1 .

The task force divided arrhythmias into three categories: shockable, nonshockable, and intermediate (Table 2 ). Patients with shockable rhythms (VF, rapid VT) potentially receive the greatest benefit (survival) from defibrillation at essentially no risk. High sensitivity for AED analysis (Table 3 Clearance Recommend Free Shipping Outlet Locations Sandals for Women On Sale White Leather 2017 45 55 Sigerson Morrison Marketable Sale Online KetCDN
) is required for this group. Patients with nonshockable rhythms derive no benefit from defibrillation and are at maximum risk. For reasons of safety, asystole is included in this group. High specificity is required. Patients with intermediate rhythms are unlikely to derive benefit or be at risk from defibrillation, making performance requirements inappropriate. Reporting arrhythmia analysis algorithm specificity or sensitivity is sufficient for this group.

View this table:
Table 2.

Performance Goals for Arrhythmia Analysis Algorithms (Artifact Free)

View this table:
Table 3.

Calculation of Sensitivity, Specificity, and Accuracy : Rhythm Classification

Table 2 shows desired performance goals for each rhythm category. These goals reflect a consensus among the participants on ANSI/AAMI (DF39) standards. 3

Performance during developmental testing is an indication of what to expect during validation. During developmental testing of automatic rhythm analysis systems, the performance goal should be met or exceeded. This maximizes chances of equaling or exceeding the goal during validation testing, which should be performed with at least the minimum sample size per category given in Table 2 . (A sample consists of data required to make a single shock/no-shock decision.) The sizes selected in Table 2 reflect a balance between reasonable confidence in performance and realistic limits on data available to demonstrate it. These are minimum sample sizes and may be exceeded. Data may be acquired from prehospital or in-hospital events. The size and geometry of the electrodes used to acquire the data should be reported.

DNA extractions from 146 individuals, that had a microsatellite DNA genotype, were selected for sequencing. Individuals that had a high assignment probability to one cluster or which showed evidence for an admixed genome were chosen. An 850 bp ND2 fragment was amplified in order to determine whether the clusters corresponded to the two mtDNA lineages outlined by Jin et al . [ 39 ]. Reactions took place in 25 ul volumes with 1 ul of DNA, 1 ul of ND2 forward and reverse primers (10 pmol ul - 1 ) (L4447 5'-aag cag ttg ggc cca tgc ccc aaa aac gg- 3' and H5622- 5'- tat ttt aat taa aat atc tga gtt gca-3'; [ Womens P Vallarta 6550732c2v16 Heels Sandals Pikolinos Discount Release Dates Free Shipping Reliable Clearance Low Price Fashion Style Cheap Price Ke6hhBu8t
]), 2 ul dNTP (10 mM of each dNTP; Roche Diagnostics), 1.5 ul Mg 2 + (25 mM), 2.5 ul 10× buffer (Takara), and 0.25 ul Taq polymerase (Takara). Thermal cycling was performed with an initial denaturation at 95°C for 5 minutes followed by 30 cycles of 95°C for 30 seconds, 50°C annealing for 30 seconds, 72°C for 45 seconds and a final extension of 72°C for 5 minutes. All PCR products were run on 1% agarose gels and purified using QIAquick PCR purification kits (Qiagen). The cleaned products were directly cycle sequenced in the forward direction using the L4447 primer. All DNA sequencing reactions were performed using BigDye terminator sequencing chemistry with an ABI 3730 sequencing machine (Applied Biosystems). Sequences were visualized and corrected using Sequencher version 4.5 (Genecode Corp) and aligned using MacClade version 4.08 [ 67 ].

A phylogenetic tree was generated to determine whether the data recovered the same clades as found by Jin et al . [ 39 ], and to assess whether the mtDNA lineages correspond to genotypic clusters found using the nuclear DNA. A Bayesian inference approach using MrBayes version 3.2 [ 42 ] was used. Akaike's Information Criterion (AIC) in MrModeltest version 2.1 [ 68 ] was used to determine the best-fit model. We used a "flat" prior and four Markov Chain Monte Carlo (MCMC) chains with 10,000,000 generations to ensure convergence. Tracer version 1.4 [ 69 ] was used to plot likelihood values and determine whether the Markov chains had reached convergence. Trees were sampled every 500 generations and the last 5,000 trees were used to estimate the consensus tree and Bayesian posterior probabilities. All prior trees were designated as "burn-in".

Acknowledgements

The authors would like to thank J. Bogart, T. Nudds, R. Danzmann, E. Timusk, K. Bi, V. Thomas and four anonymous reviewers for insightful comments and suggestions on earlier versions of this manuscript. We would also like to thank K. Bi and A. Zhan for technical help in the lab. All animal processing followed approved protocols from the University of Guelph. Support for this project came from a NSERC (Canada) discovery grant to JF.

12862_2009_1413_MOESM1_ESM.pdf Authors’ original file for figure 1
12862_2009_1413_MOESM2_ESM.pdf Authors’ original file for figure 2
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Authors’ original file for figure 3
12862_2009_1413_MOESM4_ESM.pdf Authors’ original file for figure 4
12862_2009_1413_MOESM5_ESM.pdf Authors’ original file for figure 5
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Authors’ original file for figure 6

DN collected and analyzed the data, and drafted the manuscript. JF conceived and designed the study, collected the samples and helped with data analysis and interpretation. YQ collected the samples and helped with drafting of the manuscript. All authors read and approved of the manuscript.

( XVIII 556–560)
And there in the midst the king, in silence, scepter in hand at the head of the reaping-rows, stood, rejoicing in his heart. And off to the side, beneath an oak, the heralds were setting out the feast, they were dressing a great ox they had slaughtered , while women poured out white barley, generous, for the reaper’s midday meal.
( XXIV 123–125)
[Thetis] found him groaning hard. Around him trusted comrades busily swung to the work, preparing breakfast, for them a large fleecy sheep lay slaughtered in the shelter.
The individual context of ἱερεύειν is important for the poem’s characterization of Agamemnon and of his the quarrel with Akhilleus. Its meanings can range from “sacrifice animals to the gods as a gesture of power” to “kill animals to eat them without pre-kill rites as an expression of deviance from expected behavior,” [ 184 ] but it is through the recollection of other scenes in the poem that ἱερεύειν generates audience expectations of animal sacrifice, expectations to be either fulfilled or frustrated according to the thematic needs of the context. When the expectation of sacrifice is introduced, if the verb is used without reference to the gods or other sacrificial details, the audience notices the of sacrifice to the gods. This destabilization of audience expectation heightens the significance of the descriptions of Akhilleus’ shield and his uneaten breakfast as divergent from Agamemnon’s animal sacrifices.
σφάζειν has a similar range of applications. This verb is also attested in Linear B: ( ), occurring on tablets from Knossos. [ Sneakers for Women On Sale Black Fabric 2017 EUR 38 UK 5 USA 7 Nike Buy Cheap Reliable bX0wTknxz
]
The and Cunliffe’s define σφάζειν as ‘slaughter’ in Homer, but it becomes ‘sacrifice’ in later authors, for example Pindar and Xenophon, and in tragedy. In tragic poetry, but not in Homer, σφάζειν can be applied to the killing of people. [ 186 ] In the Classical period, it is even possible to broadly categorize different types of sacrifice according to the use of this verb: sacrifices are followed by a feast on the basis of the frequent use of the verb θύειν, and sacrifices are uneaten on the basis of the use of the verb σφάζειν, which refers only to the kill and need not be followed by sacrifice. Thus Casabona believes that σφάζειν, with a few exceptions, represents an uneaten sacrifice, as seems to be its meaning in the Classical period. [ 187 ] As opposed to the happy contexts for feasting provided by most sacrifices, sacrifices are performed in the Classical period at the taking of oaths, in purification rites, and before battle for divination purposes. [ 188 ]
ἔσφαζον θαλέθοντες ἀλοιφῇ εὑόμενοι τανύοντο διὰ φλογὸς Ἡφαίστοιο
( IX 465–469)
Holding me in the house, begging me to stay, they butchered plenty of fat sheep, and shambling crook-horned cattle, droves of pigs, succulent, rich with fat— they singed the bristles, splaying them out across Hephaistos’ fire , then a great amount of wine was poured from the old man’s jars.
This description is part of Phoinix’s autobiographical story of betrayal and withdrawal: he was persuaded to sleep with his father’s concubine by his jealous mother, and after ‘one of the immortals’ stops him from killing his father, he decides to leave home ( IX 444–463). There are numerous striking parallels between Phoinix’s experience and Akhilleus’ own wrath and withdrawal, but the object of his speech is to persuade the young hero to heed his advice not to abandon the Akhaian army. [ 189 ] The description of Phoinix’s friends and relatives beseeching him and killing numerous animals is not meant to imply sacrifice, since there are no references to divinities or pre-kill rites. Therefore, it belongs to a different end of the spectrum of meaning implied by ἔσφαζον than the commensal feasts arranged by Agamemnon. Phoinix’s apparent lack of participation in this feast may anticipate Akhilleus’ thematic opposition to sacrifice, which will become so important in the latter half of the poem. He goes on to describe the power of sacrifice in his paradigm of the and in Oineus’ neglected sacrifices to Artemis, to which we will return below. Phoinix presents a range of eating and sacrificial activities, all anecdotes about the anger of gods: Artemis is angry about neglected sacrifices; the anger of the gods can generally be appeased by sacrifices. His speech is, of course, unsuccessful, and Akhilleus remains withdrawn.
“ἀλλ’ ἄγε δὴ καὶ νῶι μεδώμεθα, δῖε γεραιέ,

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